Sterilization of Liquid Tanks

Sterilization of liquid tanks is crucial for removing microorganisms, chemicals, and particulate matter from the liquids before and after use. Devices suitable for working with different chemicals such as alcohol, oil, water, and antifreeze play a critical role in many scientific research and industrial processes. These devices are used for processes like conditioning liquids. However, during operation, it is inevitable that different chemicals will mix with these liquids. Additionally, regularly renewing these liquids is important to ensure the accuracy of the work. For these reasons, cleaning and sterilizing liquid tanks is of great importance.
In this article, we will discuss various methods that can be applied for the sterilization of Labo devices and general-purpose liquid tanks used in industries, as well as points to consider.

 

Importance of Sterilization

• Prevention of Contamination: Sterilization ensures the complete removal of microorganisms and contaminants from inside the device, increasing the reliability of subsequent experiments.
• Prolonging Device Lifespan: Regular sterilization prevents biofilm formation inside the device and extends its lifespan.
• Safety: A sterile working environment protects the health of laboratory personnel and reduces potential infection risks.

Sterilization Methods

1. Heat Sterilization

   • AutoclavingThis method uses steam under high temperature and pressure to eliminate microorganisms. It can be suitable for tanks containing mixtures of alcohol, oil, and water, but the heat resistance of chemicals like antifreeze should be checked.
   • Dry Heat Sterilization: This method uses dry heat at high temperatures and is suitable for heat-resistant materials sensitive to moisture. However, longer periods may be needed to completely remove organic materials.

1. Chemical Sterilization

   • Glutaraldehyde: A broad-spectrum biocide, glutaraldehyde provides a high level of sterilization. However, it is toxic and must be used with care.

   • Hydrogen Peroxide: An environmentally friendly sterilant that can be used in vapor or plasma form. However, it may react with some organic substances.

   • Ethylene Oxide: Ethylene oxide, known for its high penetration capability, is suitable for heat-sensitive materials. However, it is a toxic and flammable gas.

2. Radiation Sterilization

   • Gamma Rays: With high penetration power, gamma rays are used to sterilize large-volume materials, but it is an expensive method.

   • Electron Beams: With lower penetration power, electron beams are suitable for surface sterilization.

3. Filtration: During operation, chemicals and substances mixed into the liquid can alter its properties. Filtration allows the removal of microorganisms and chemicals without damaging the liquid's temperature or the structure of its components.

Advantages of Filtration

• Protection of Sensitive Components: It does not damage the structure of heat-sensitive molecules like proteins and enzymes.

• Fast and Effective: Sterile liquid can be obtained in a short time.

• Versatile Use: It can be used for liquids of different volumes and viscosities.

Filters Used in Filtration

• Membrane Filters: Typically made from materials such as cellulose ester or polyether sulfone. The pore sizes are selected based on the size of the particle to be filtered.

• Depth Filters: Consist of beds made of glass beads, soil, or other inert materials. Effective in trapping larger particles.

Disadvantages of Filtration:

• Filter Clogging: Filters can become clogged in liquids with a high particle load.

• Filter Cost: Filters used for high flow rates can be costly.

Considerations During Filtration:

• Filter Selection: The appropriate filter should be selected based on the viscosity, particle size, and flow rate of the liquid.

• Sterile Working Conditions: The environment should be sterile during the filtration process.

• Filter Validation: The effectiveness of the filters should be regularly checked.

Sterilization Process

• Pre-cleaning: Before sterilization, the liquids in the tank should be completely drained, and the tank should be mechanically cleaned.
• Selection of Sterilant: The sterilant to be used should be chosen based on the tank material, type of contamination, and sterilization objectives.
• Setting Parameters: Parameters such as sterilization time, temperature, pressure, and sterilant concentration should be adjusted according to the chosen method and the properties of the sterilant.
• Validation: Validation studies should be conducted to confirm the effectiveness of the sterilization process.
• Drying: In heat sterilization, it is important to ensure that materials are completely dried after the sterilization process.

Points to Consider

• Material Compatibility: Ensure that the sterilant is compatible with the tank material.
• Removal of Residue: Care should be taken to ensure no sterilant residue remains in the tank after sterilization.
• Safety: Safety precautions should be followed during sterilization processes.

• Documentation: Sterilization processes should be thoroughly documented.

Sterilization of liquid tanks containing working fluids such as alcohol, oil, water, and antifreeze is important for prolonging the device's lifespan, preventing contamination, ensuring accurate test results, and maintaining a safe working environment. The sterilization method should be chosen based on the tank material, type of contamination, and sterilization goals. Safety measures must be followed during the sterilization process, and the procedures should be carefully documented.

For detailed information about liquid tank sterilization for both industrial tanks and Labo devices, you can easily contact our project engineers at info@labo.com.tr or by calling +90 216 329 11 77.